Temporary
Part:BBa_K1017402:Design
Designed by: Wen-Hui Cheng Group: iGEM13_NCTU_Formosa (2013-09-12)
sRNA+rRBS-2
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 97
Illegal SpeI site found at 82 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 82
Illegal NotI site found at 88 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 3
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 97
Illegal SpeI site found at 82 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 97
Illegal SpeI site found at 82 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Included the sRNA-2 we designed and its complement DNA sequence to produce RBS binding site. We can use this two complement parts to control on or off of other operons.
Source
synthesis