Temporary

Part:BBa_K1017402:Design

Designed by: Wen-Hui Cheng   Group: iGEM13_NCTU_Formosa   (2013-09-12)

sRNA+rRBS-2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 97
    Illegal SpeI site found at 82
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 82
    Illegal NotI site found at 88
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 97
    Illegal SpeI site found at 82
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 97
    Illegal SpeI site found at 82
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Included the sRNA-2 we designed and its complement DNA sequence to produce RBS binding site. We can use this two complement parts to control on or off of other operons.


Source

synthesis

References